"Jed" wrote:
 
>I was wondering how does one convert BioRad *.pic files to GIF format?
 
What puzzles me is why anyone would want to!
GIF is a "compressed" format - ie it tries to compress images BUT
confocal images are generally not very compressible and when you
convert them to GIF they can actually get bigger! I tried this out
on one of my standard test images - a Bio-Rad image of a silicon
chip which hasd been cropeed to 640 x 480.  As an uncompressed TIFF
file its size was 309,432 bytes.  Converted to a GIF file it was
331,815 bytes!
 
So my Parfocal software does NOT convert to GIF, and I'd suggest that
TIFF is a much more sensible format for general purpose file exchange.
(Of course you can convert to TIFF or BMP with Parfocal then use a
public domain or shareware conversion to get GIF, which is what I did.)
 
 
On a separate point, do other Bio-Rad users find that they commonly
get 'glitches' in side-by-side 2-channel images?  It is a bit of a
problem here.  I have actually written a 'deglitching' program
(which I'm happy to make available by ftp if there is any interest)
but I'd much rather get rid of the problem at the source.
 
                        Guy Cox
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