Dear friends,
I have recently biught the Nikon 40x, NA=1,3, oil immersion: it is
spectacular  both for conventional and two-photon excitation.
Bye
Alby
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Alberto "Alby" Diaspro
INFM- Dept. Physics, Univ.Genoa
Via Dodecaneso 33, 16146 Genoa - Italy
voice:+39-0103536426/480
facsimile: +39-010314218
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----- Original Message -----
From: Lawrence Kordon <[log in to unmask]>
Newsgroups: bit.listserv.confocal
To: <[log in to unmask]>
Sent: Wednesday, June 14, 2000 6:40 PM
Subject: Re: objectives for MP


> Dr. Crammer,
>
> Nikon actually has an Objective made specifically for this application. It
is a
> (#93112) Plan Apochromat 100x oil, na. 1.4 with coatings (or the lack of)
> specifically for deep IR. These Objectives were also hand selected for the
"best
> of the lot" in PSF results. Also, most of the Nikon Plan Fluors and Super
Fluors
> should suffice for superior IR tranmission; as the Nikon platform is the
most
> common on the Bio-Rad Multi-Photon Systems. Mario Moronne makes a good
point in
> that all PSF's are unique to a lens. We have no problem bringing a few
"common"
> objectives out for your  PSF evaluations. For more information and
> demostrations, please contact your local Nikon Dealer: Morrell Instruments
at
> (516) 673-8777.
>
> Regards,
>
> Lawrence Kordon
> Nikon, Inc.
> Senior Bioscience Specialist
> [log in to unmask]
>
>
> Michael Cammer wrote:
>
> > We are planning the configuration for a multi-photon microscope.  Does
> > anybody have knowledge/opinions about
> > 1.  IR coating/correction in Nikon vs. Olympus optics; and
> > 2.  PSF (obviously only important in this application for delivery of
> > excitation) in Nikon vs. Olympus optics.
> > Thanks-
> >
> >
____________________________________________________________________________
> > Michael Cammer   Analytical Imaging Facility   Albert Einstein Coll. of
Med.
> > 1300 Morris Park Ave.    Bronx, NY  10461    (718) 430-2890   Fax:
430-8996
> > URL:  http://www.aecom.yu.edu/aif/
> > personal:  http://cammer.home.mindspring.com/
>