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Glen,
It sounds like the objective is contacting your slide. What is the objective that you are using? I can tell you what the working distance is.
Michelle
Michelle Ocana
Confocal and Imaging Specialist
Optical Analysis Corporation
3 Bud Way, Suite 25
Nashua, NH 03063-1700
c: 617-447-4391
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Glen MacDonald
Sent: Tuesday, March 27, 2007 3:45 PM
To: [log in to unmask]
Subject: Image shift
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Hi,
I'm trying to diagnose an odd problem with an FV-1000 and IX-81
microscope, to get speed things in getting this serviced. When
collecting a z-series, around 330 to 400 µm into the volume, the
image shifts in the x-axis by several pixels to the left, sometimes 1
or 2 lines upwards. The shift may occur within 1 line, or up to 15
lines resembling a vibration, but trending to the left. in the
subsequent portion of the stack, additional shifts will occur every
40 to 120 µm. If I capture the full volume in increments of less
than 400 µm, the shift will still occur. I've used box sizes from
256 to 1024 at dwell times of 2 µs to 20 µs. At 2 µs with a 256x256
box, the shift doesn't occur within a frame, but seems to be between
the frames. Also, the shift always occurs in the upper 1/3 of an image.
In line sequential acquisition, the shift appears in all channels.
With sequential frame acquisition, the shift appears within a frame
of the first channel but the slice images for the other channels
shift in their entirety.
The air table is up, although vibrations shouldn't be this regular.
Also, the starting point of the z-series may vary, relative to the
coverslip.
Any suggestions?
Regards,
Glen
Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923 USA
(206) 616-4156
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The box said "Requires WindowsXP or better", so I bought a Macintosh.
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