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March 2008

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Subject:
dyes
From:
Nicole Young <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 18 Mar 2008 13:30:37 -0500
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I am tryring to view an eukaryotic cell infected with amoeba and I am using BacLight for the bacteria but it is also staining the cytoplasm of my amoeba green making it difficult to determine the location of the live bacteria.  Can anyone recommend a dye that would be better or a dye that would stain the amoeba a different color other than green?  The lasers I have to work with range from 457 to 639 so DAPI and Hoescht are out.

Nicole Young

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