Dominique Miller wrote:
>
> Hi all
>
> Interesting discussion about 3D software.  It is a shame though that
> very few people seem to be able to distinguish between 3D software and
> [...]
> How many people out there actually know the difference between 2.5D and
> 3D?  Please let me know I would really be interested.

Hello, Dominique.

I'm sure I'm not the only person to point out to you that the images are
three-dimensional, not the software ...

Nevertheless, you make an important point: I've been doing 2.5-D image
analysis for a while and it is not good enough unless, as in my case,
the structure of your samples is such that interpolation between
sections is reasonably justified. I'm working on quantitation of plant
stem anatomy and, along the longitudinal axis, the sclerenchyma (fibre)
cells are reasonably well represented by interpolating between
transverse optical sections a few microns apart. However, we ran into
real problems when obliqely sectioning parenchyma cells!

        Tony.
--
Dr. A.J.Travis,                     |  mailto:[log in to unmask]
Rowett Research Institute,          |    http://www.rri.sari.ac.uk/~ajt
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