Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi Amber, 

Gert's suggestion works well if you have the proper software version.  I 
find the whole process of defining and saving ROIs to be cumbersome and 
non-intuitive.  A simple way around the problem is to draw a shape over 
the ROI using the drawing tools in the overlay tab.  When you save the 
image, the shape is saved and can be viewed with the LSM Reader software.

Good luck, John.

Amber Bruns wrote:

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Hello,
>I am a research associate at the University of North Dakota and we are
>currently using a 510 LSM META confocal microscope from Zeiss to preform
>FRAP.While doing FRAP, I define a region of interest to bleach out,then after
>FRAP is completed the image is saved with the  defined ROI still showing. Later
>I tried to  open up the image from a different computer which has the LSM
>software,and the ROI outline is gone. I tried this several times and could
>never get the ROI to show up .I was wondering if anybody had a trick to save
>the ROI along with the FRAP series so that when you opened up you image from a
>different computer your defined ROI was present.
>
>Amber Bruns
>Research Associate
>UND Medical School
>
>  
>

-- 

*********************************
C. John Runions, Ph.D.
School of Biological and Molecular Sciences
Oxford Brookes University
Oxford, UK
OX3 0BP

email: [log in to unmask]
phone: +44 (0) 1865 483 964