Dear Dr. Schmid,

We use the indirect tyramide kit of NEN Life Sci.
It works good and we observe fluorescence signals with low background on
plant chromosomes.
But.....If the antibody is bad, replace to good one?

S.Nakayama

At 1:31 PM -0800 01.2.28, alice schmid wrote:
>  Dear List,
>
>      I am trying to get tyramide reagents to work in
>  fly embryos...we have a bad antibody and I've been
>  told it is a way of increasing specificity of your
>  signal.  Does anyone have recommendations?  Previous
>  attempts have yielded high background.  I have tried
>  preabsorbing the antibody, but seem to be getting high
>  background just with the tyramide reagents by
>  themselves (i.e., no primary (negative controls)).  So
>  I need input on how to fine tune it in flies!  Any and
>  all suggestions welcome.
>
>                                          Alice Schmid
>                                          Caltech
>
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