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Hello,
We have analysed with success cells coming from Europe and the States that
were cultured on coverslips and fixed with para.
Coverslips were put into individual 50ml conical tubes (retrieval would be
difficult in 15 ml) filled with buffer and then closed with parafin + cap +
parafin. They were then shipped in an isolated box cooled (not frozen) with
dry ice. A small amount of fixator can be added to the buffer if needed.
With proper sealing, coverslip could probably be sent in multiwell as well,
eliminaing the need for the identification of the coverslip side with cells.
M
Marc Thibault, Ph.D.
Posdoctoral Fellow
Canada Chair in Cartilage Tissue Engineering
Department of Chemical Engineering
Ecole Polytechnique
Montreal, Qc, Canada
-----Message d'origine-----
De : G. Esteban Fernandez [mailto:[log in to unmask]]
Envoyé : 5 mars 2007 12:31
À : [log in to unmask]
Objet : Shipping cells on coverslips in 6-well plates
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Hi all,
A user needs to ship mammalian cells grown on coverslips in 6-well
plates and fixed with methanol. Any suggestions on how to do this so
the cells don't detach during shipping?
Thanks.
--
G. Esteban Fernandez, Ph.D.
Associate Director
Molecular Cytology Research Core Facility
University of Missouri-Columbia
(573) 882-4895
http://www.biotech.missouri.edu/mcc/
