Has there been any validation experiment done to determine if there is
compression of thick tissue specimens (100-200 microns) during confocal
imaging with oil-immersion lenses (40/60/100X)?  I am imaging spinal cord
sections of 150 microns, and the neurons in them are distributed all across
the thickness of a section.  I am interested in determining if the estimates
of thickness that I get for neurons on one side of the section are affected
by any compression of the tissue by the advancing objective during data
collection.
 
Any input is greatly appreciated.
 
 
                                Y.S. Prakash