Monica K. Chawla, Gang Lin, Kathy Olson, Almira Vazdarjanova, S. N. Burke, B. L. McNaughton, P. F. Worley, John F. Guzowski, Badrinath Roysam and C. A. Barnes, “3D-catFISH: A System for Automated Quantitative Three-Dimensional Compartmental Analysis of Temporal Gene Transcription Activity Imaged by Fluorescence in situ Hybridization,” Journal of Neuroscience Methods, 139, 13–24, 2004.
John F. Guzowski, Jerilyn A. Timlin, Badri Roysam, Bruce L. Mc Naughton, Paul F. Worley and Carol A. Barnes, “Mapping Behaviorally Relevant Neural Circuits With Immediate Early gene Expression,” Current Opinion in Neurobiology, Vol 15, Issue 5, 2005.
Vicki L Sutherland, Jerilyn A Timlin, Linda T Nieman, John F Guzowski, Monica K Chawla, Paul F Worley, Badri Roysam, Bruce L McNaughton, Michael B Sinclair, Carol A Barnes, “Advanced Imaging of Multiple mRNAs in Brain Tissue using a Custom Hyperspectral Imager and Multivariate Curve Resolution,” Journal of Neuroscience Methods, Feb 15;160(1):144-8, 2007.
Thanks,
Badri Roysam
Professor, Department of Electrical, Computer and Systems Engineering
Associate Director, NSF Center for Subsurface Sensing & Imaging Systems (CenSSIS ERC)
Co-Director, Rensselaer Center for Open Source Software
Rensselaer Polytechnic Institute
110 8th Street, Troy, New York 12180-3590, USA.
Office(JEC 7010): 518-276-8067, Assistant: 518-276-8525, Lab(JEC 6308): 518-276-8207, Fax: 518-276-8715
Email: [log in to unmask], Web: http://www.ecse.rpi.edu/~roysam
----- Original Message -----
From: Keith Morris [mailto:[log in to unmask]]
To: [log in to unmask]
Subject: Re: labeling cell nuclei
> Hi Badri,
>
>
>
> I assume we are talking molecular cytogenetics. Often confocals aren't the
> first choice at imaging FISH [fluorescent in-situ hybridization] metaphase
> chromosomes & interphase nuclei, although we do use ours for 3D FISH, i.e.
> looking at chromatin organization in the nuclei in, naturally, 3D [see
> Farids post]. Otherwise most molecular cytogenetics systems involve 100x
> [even 150x] high NA oil objectives and a standard wide-field upright
> fluorescence microscope [with multiple narrow band fluorescence filter
> sets]. These microscopes are controlled via specialized cytogenetics
> software* for karyotyping, CGH, mFISH etc... The nuclei/chromosomes are
> typically stripped of the surrounding cell cytoplasm and hence quite flat
> [and the structures are very small]. Imaging is via specific fluorescence
> chromosome/centromere/telomere/arm paints and probes targeted to small
> sequences of DNA. My line manager [or 'boss' as we used to say] is in fact
> in the process of editing a large tome on all things FISH related, and this
> should be published early next year.
>
>
>
> Regards
>
>
>
> Keith
>
>
>
> Links to molecular cytogenetics websites
> http://www.well.ox.ac.uk/cytogenetics/websites.shtml
>
> Reading list
> http://www.well.ox.ac.uk/cytogenetics/reading.shtml
>
>
>
>
> *e.g. Applied Imaging [Genetix]
> http://www.genetix.com/en/systems/cytovision/introduction/index.html
> Metasystems
> http://www.metasystems.de/indexold.htm
>
> Spectral-imaging
>
> http://www.spectral-imaging.com/spectral.asp
>
>
>
>
>
>
>
> ---------------------------------------------------------------------------
> Dr Keith J. Morris,
> Molecular Cytogenetics and Microscopy Core,
> Laboratory 00/069 and 00/070,
> The Wellcome Trust Centre for Human Genetics,
> Roosevelt Drive,
> Oxford OX3 7BN,
> United Kingdom.
>
> Telephone: +44 (0)1865 287568
> Email: <mailto:[log in to unmask]> [log in to unmask]
> Web-pages: <http://www.well.ox.ac.uk/cytogenetics/>
> http://www.well.ox.ac.uk/cytogenetics/
>
> From: Confocal Microscopy List [mailto:[log in to unmask]] On
> Behalf Of Farid Jalali
> Sent: 10 July 2009 22:34
> To: [log in to unmask]
> Subject: Re: labeling cell nuclei
>
>
>
> Hello Badri,
>
> A couple of these are quite all-encompassing. The article by Wojcik and
> Dobrucki will be of particular interest if you are looking at chromatin
> dynamics and processes that impact chromatin structure.
>
> Hope it helps.
> Cheers
> Farid
>
> 1. Stuart KR and ES Cole. Nuclear and cytoskeletal fluorescence microscopy
> techniques. Methods in Cell Biology (2001). 62:291-311
>
> 2. Ploeger LS, Dullens HF, Huisman A, van Diest PJ. Fluorescent stains for
> quantification of DNA by confocal laser scanning microscopy in 3-D.
> Biotechnic Histochemistry (2008). 83(2):63-9.
>
> 3. Suzuki T, Fujikura K, Higashiyama T, Takata K. DNA Staining for
> fluorescence and laser confocal microscopy. Journal of Histochemistry and
> Cytochemistry (1997). 45(1):49-53.
>
> 4. Wojcik K and JW Dobrucki. Interaction of a DNA intercalator DRAQ5, and a
> minor groove binder SYTO17, with chromatin in live cells--influence on
> chromatin organization and histone-DNA interactions. Cytometry A (2008).
> 73(6):555-562.
>
>
>
>
> On Fri, Jul 10, 2009 at 9:05 AM, Badri Roysam <[log in to unmask]> wrote:
>
> Dear Colleagues, I am looking for a comparative review of methods for
> fluorescently labeling cell nuclei
> for confocal imaging. Has anyone written such a review?
>
> Thanks for any pointers,
>
>
> Badri Roysam
> Professor, Department of Electrical, Computer and Systems Engineering
> Associate Director, NSF Center for Subsurface Sensing & Imaging Systems
> (CenSSIS ERC)
> Co-Director, Rensselaer Center for Open Source Software
> Rensselaer Polytechnic Institute
> 110 8th Street, Troy, New York 12180-3590, USA.
> Office(JEC 7010): 518-276-8067, Assistant: 518-276-8525, Lab(JEC 6308):
> 518-276-8207, Fax: 518-276-8715
> Email: [log in to unmask], Web: http://www.ecse.rpi.edu/~roysam
> <http://www.ecse.rpi.edu/%7Eroysam>
>
>
>
>
|
