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On a related note:

Bromophenol blue (BPB) was used at 0.5 - 2 mM to quench extracellular GFP
fluorescence from fusion proteins (see Harata... Tsien Neuron 2006 and
Song... Poo Cell 2009). However in our hands (we tried only once), it lead
to a dramatic photosensibilization of cultured neurons, they would just die
very rapidly under reasonable imaging conditions, and we weren't able to
use this method. I'd be happy to hear about people that successively used
BPB for this purpose.

--
Christophe Leterrier
Researcher
Axonal Domains Architecture Team
CRN2M CNRS UMR 7286
Aix Marseille University, France



On Fri, Jun 7, 2013 at 1:03 PM, MODEL, MICHAEL <[log in to unmask]> wrote:

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>
> I think trypan blue has also been used to quench external fluorescence
> ________________________________________
> From: Confocal Microscopy List [[log in to unmask]] on
> behalf of Gabriel Lapointe [[log in to unmask]]
> Sent: Thursday, June 06, 2013 5:17 PM
> To: [log in to unmask]
> Subject: Re: How to show something inside the cell?
>
> *****
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>
> Hi Yi,
> In the same line of thougth as Mike, you could also look at the quencher
> used in qPCR. Some might be compatible with your fluorophore.
>
>
>
> *Gabriel Lapointe, M.Sc.*
> Lab Manager / Microscopy Specialist
> Concordia University, Biology Department
> 7141 Sherbrooke St. West SP 534
> Montréal QC H4B 1R6 Canada
> Lab : (514) 848-2424 x5988
> Office : (514) 848-2424 x3008
> Fax : (514) 848-2881
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> [log in to unmask]
> cmac.concordia.ca
> http://gabriellapointe.ca
>
>
> 2013/6/6 MODEL, MICHAEL <[log in to unmask]>
>
> > Hi Yi - I think your solution is good. Fluorophores on the surface can
> > also be selectively quenched, depending on the fluorophore - FITC by
> > lowering pH, red fluorophores or those emitting in the 600s by adding
> > several mg/ml of Acid Blue 9.
> >
> > Mike Model
> >
> >
> > -----Original Message-----
> > From: Confocal Microscopy List [mailto:[log in to unmask]]
> > On Behalf Of SUBSCRIBE CONFOCALMICROSCOPY
> > Sent: Thursday, June 06, 2013 4:27 PM
> > To: [log in to unmask]
> > Subject: How to show something inside the cell?
> >
> > *****
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> >
> > Dear listers,
> >
> > One of our user is investigating the transportation of fluorescence
> > labeled DNA oligo in cells. He would like to prove the DNA oligo has been
> > transported inside the cells but not on the surface of cells by using the
> > light microscopy. We suggested him to clearly label the membrane of cells
> > and use the Z-stack images of confocal to look through the cells.
> > I am wondering if there is any other better solution?
> >
> > Any suggestion on good membrane dye is appreciated as well!
> >
> > Thank you,
> >
> > Yi
> >
> >
> >
>
>