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Not with confocal, but yes with multiphoton with an open beam path. Wavelengths somewhere in the range of 820 to 960 nm. Both an injury and black spots on the wall.
According to a quick google search, commercial CMOS cameras are susceptible to laser damage too.
===========================================================================
Michael Cammer, Microscopy Core & Dustin Lab , Skirball Institute, NYU Langone Medical Center
Cell: 914-309-3270 Lab: 212-263-3208
http://ocs.med.nyu.edu/microscopy & http://www.med.nyu.edu/skirball-lab/dustinlab/
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Indig, Fred (NIH/NIA/IRP) [E]
Sent: Friday, October 25, 2013 3:37 PM
To: [log in to unmask]
Subject: Re: Laser Safety Issues
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Just out of curiosity, is anyone aware of an accident or injury involving the laser of a confocal LSM?
Fred E. Indig, Ph.D.
Head, Confocal Imaging Facility
Biomedical Research Center (BRC) Rm 8B135 National Institute on Aging/NIH
251 Bayview Bld.
Baltimore, MD 21224-6825
Tel. 410-558-8173
Fax 410-558-8236
[log in to unmask]
________________________________________
From: Neeraj Gohad [[log in to unmask]]
Sent: Friday, October 25, 2013 3:23 PM
To: [log in to unmask]
Subject: Re: Laser Safety Issues
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You are welcome Andrea, it's a good resource and our laser safety officer was quite happy that I followed the recommendations in it. If there are extensive custom setups, our Univ. also has access to Laser Safety in Educational Institutions Training -online course from LIA.
Best Regards,
Neeraj.
Neeraj V. Gohad, Ph.D.
Assistant Research Professor
Vice President: Sigma Xi, Clemson Univ. Chapter Department of Biological Sciences
132 Long Hall
Clemson University
Clemson,SC-29634
Phone: 864-656-3597
Fax: 864-656-0435
http://www.clemson.edu/cafls/departments/biosci/faculty_staff/gohad_n.html
http://www.clemson.edu/sigmaxi/index.html
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Elberger, Andrea June
Sent: Friday, October 25, 2013 3:13 PM
To: [log in to unmask]
Subject: Re: Laser Safety Issues
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NEERAJ - Thanks for the link to the ANSI site, it will be a useful reference point for us.
ANDREA
Andrea J. Elberger, Ph. D.
Professor, Anatomy and Neurobiology
Director, Confocal Laser Scanning Microscope Facility The University of Tennessee Health Science Center
855 Monroe Avenue
Memphis, TN 38163 U.S.A.
tel: 901-448-4101
FAX: 901-448-7193
<mailto: [log in to unmask]>
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Neeraj Gohad
Sent: Thursday, October 24, 2013 6:57 PM
To: [log in to unmask]
Subject: Re: Laser Safety Issues
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Hi Andrea,
Might also want to check out the link below. The ANSI standard has some good pointers as well.
https://www.lia.org/store/ANSI/114
ANSI Z136.5 - Safe Use Of Lasers In Educational Institutions (2009)
Best,
Neeraj.
Neeraj V. Gohad, Ph.D.
Assistant Research Professor
Vice President: Sigma Xi, Clemson Univ. Chapter Department of Biological Sciences
132 Long Hall
Clemson University
Clemson,SC-29634
Phone: 864-656-3597
Fax: 864-656-0435
http://www.clemson.edu/cafls/departments/biosci/faculty_staff/gohad_n.html
Sent from my iPhone
On Oct 24, 2013, at 3:34 PM, "Elberger, Andrea June" <[log in to unmask]<mailto:[log in to unmask]>> wrote:
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TO THE CONFOCAL LIST MEMBERS:
I am a member of a campus-wide Laser Safety Subcommittee, and we are trying to establish policies and standards for numerous systems (more than microscopes) that use lasers for research. In the case of Confocal Laser Scanning Microscope systems, all those on our campus are commercially available systems.
I am seeking information on what CLSM facilities have done, or tried to do, related to laser safety policies and issues.
For example:
1) What training requirements/procedures do you have for trainers and end-users?
2) Do you have any modifications to your existing commercial systems, e.g. a screen around the microscope or stage where the laser beam is visible?
3) Do you require users to wear protective eyewear at any time during operation of the CLSM?
4) Do you have the manufacturer's service people carry out any measurements that would be used in laser safety calculations?
Any information on other procedures or issues related to use of the lasers in CLSMs would be greatly appreciated.
Thanks for your time.
ANDREA
Andrea J. Elberger, Ph. D.
Professor, Anatomy and Neurobiology
Director, Confocal Laser Scanning Microscope Facility The University of Tennessee Health Science Center
855 Monroe Avenue
Memphis, TN 38163 U.S.A.
tel: 901-448-4101
FAX: 901-448-7193
<mailto: [log in to unmask]<mailto:[log in to unmask]><mailto:[log in to unmask]<mailto:[log in to unmask]>>>
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