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Dear Amandeep,

Previous discussions have attributed autofluorescence to the riboflavin in
the DMEM and possibly the folic acid and phenol red in that and other
culture media:

http://confocal-microscopy-list.588098.n2.nabble.com/fluorescent-medium-td1691899.html
and
http://www.bio.net/bionet/mm/fluorpro/1999-February/001193.html

Depending on your experimental constraints you can try different media, as
suggested in these threads, with less riboflavin. A filter set that can
suppress it is also suggested.

Good luck,
Graham

-- 
*Graham Wright, PhD*
Microscopy Unit Manager

Institute of Medical Biology
8A Biomedical Grove, #06-06 Immunos, Singapore 138648

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On 4 June 2012 14:11, Amandeep Walia <[log in to unmask]> wrote:

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> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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>
> Dear All
>
> Can any one tell me why the Hela cells gives flourescence when the are
> grown with DMEM media and when they are seen under 488 and 543 lasers they
> give flourscence. what to do ??
>
> Regrads
> Amandeep walia
>