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Dear Mike,

because the 458 line  was a weak line of the Argon laser with less than 5%
of the total output power, for GFP excitation there were often limitations
to the excitation power, in particular when using low NA lenses and as a
consequence, also filter manufacturers offered less options including 458
in multiline dichroics.

So if you can provide a powerful enough laser -also to compensate for the
smaller excitation cross section- and suitable excitation optics, 458
should be well suited for GFPturbo excitation.

Greetings, Jens


On Tue, May 6, 2014 at 7:05 PM, MODEL, MICHAEL <[log in to unmask]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Colleagues,
>
> We need to buy optics to do mGFP-turbo RFP FRET, and it seems that
> excitation at 458 might give much less direct excitation of RFP with only
> little sacrifice in GFP excitation. But there might be some other
> consideration of which I am unaware. Has anyone had any good/bad experience
> using 458 line for GFP? Thanks in advance!
>
> Mike Model
>