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I suggest staining the amoebae with one of the cytoplasmic CellTracker
dyes - probably CellTracker Orange CMTMR to give good spectral
discrimination from the SYTO 9 (BacLight)dye.  Also cutting the staining
concentration or incubation time for the SYTO 9 dye will imnprove its
discrimination of the bacteria over the amoebae.

Full disclosure: SYTO 9 and CellTracker Orange CMTMR are
Invitrogen/Molecular Probes products.

Iain Johnson
Invitrogen Corp.

-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Nicole Young
Sent: Tuesday, March 18, 2008 11:31 AM
To: [log in to unmask]
Subject: dyes

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I am tryring to view an eukaryotic cell infected with amoeba and I am
using BacLight for the bacteria but it is also staining the cytoplasm of
my amoeba green making it difficult to determine the location of the
live bacteria.  Can anyone recommend a dye that would be better or a dye
that would stain the amoeba a different color other than green?  The
lasers I have to work with range from 457 to 639 so DAPI and Hoescht are
out.

Nicole Young