Subject: | |
From: | |
Reply To: | |
Date: | Fri, 15 Jul 1994 21:59:16 +0200 |
Content-Type: | text/plain |
Parts/Attachments: |
|
|
Solution of "temperature problem" also?
When using immersion lenses in studies of living cells, one apparently need to heat the objective if the cell should sense 37C. One problem is then that the refractive index of the immersion oil is temperature dependent. Therefore it whould be nice to have different oils for different temperatures.
Maybe the oils from "Cargille's Laser Liquid, Series 5610" will do, or do you have other options out there for different temperature corrected oils?
Jason Swedlow wrote:
> For immersion oils with a range of refractive indices, we use Cargille's
> Laser Liquid, Series 5610. These are very low fluorescence, non-toxic,
> UV transparent, safe for the lenses (some oils corrode the mounting
> adhesives-- not a trivial issue, as it turns out), and you can get them
> in a range of refractive indices (1.510 - 1.5300, I think). Any oils
> within this series can be mixed to generate a desired index of
> refraction.
~~~~~~~~~~~~~~~~~~~~~~~
From: Martin Kohler
[log in to unmask]
~~~~~~~~~~~~~~~~~~~~~~~
|
|
|