Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Dear all,

I have a colleague who is doing co-cultures of 
human and mouse cells. After some days of 
co-culture and fixation, we need do determine 
whether given cells are from mouse or human origin.

So far, we applied an SC-35 spicing antibody 
which stains nicely most human but not mouse 
nuclei. Controls show however that some human 
cells are stained only very weakly. Therefore, it 
would be nice to positively identify the mouse 
cells by antibody-staining in a different color. 
The cell lineage in question is endothelial cells.

Did anybody come across an antibody (or other 
agent) that will stain mouse cells but not human cells?

Unfortunately, we cannot exploit DAPI staining 
for nuclear morphology in our setting. We could 
go for FISH with genomic or repetitive DNA but 
antibody staining would be so much faster and easier.

Thanks for any hints

Steffen

-- 
---------------------------------------------------------------------------------------------------
Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Walter-Brendel-Zentrum (WBZ)
Head of light microscopy

Building location and address for courier, parcel services etc:
Marchioninistr. 27, D-81366 München (Großhadern)

Mail room (for letters etc.):
Marchioninistr. 15, D-81366 München

Phone: +49/89/2180-76509
Fax:   +49/89/2180-76503 (please anounce incoming fax by e-mail)
skype: steffendietzel
e-mail: [log in to unmask] (for everything university related)
          or [log in to unmask]