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April 2015

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Subject:
From:
Francisco J H Blazquez <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 1 Apr 2015 11:01:56 -0300
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*****
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Hello
Can't they use another (the correct) primary antibody and use another 
chromogen? Like a double staining?
Or redo it using immunofluorescence? In this case the DAB will not be seen.

On 01/04/2015 02:08, Paul Rigby wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi All,
> One of our users has had a mishap with some very precious tumour sections which were accidentally stained with the wrong primary antibody followed by DAB. They want to know if it is possible to destain the sections (remove the DAB, which is, apparently, quite dark). They have experience removing immunofluorescence antibody staining but not the DAB product.
> All suggests will be very gratefully accepted.
> Many thanks
> Paul
>
> Assoc. Prof. Paul Rigby
> Optical Microscopy Specialist
> Centre for Microscopy, Characterisation & Analysis (M510)
> The University of Western Australia
> 35 Stirling Highway
> Crawley  WA  6007
> Australia
>


-- 
Prof. Dr. Francisco Javier Hernandez Blazquez
University of São Paulo

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