Dear Hesam,

We use the TOTO-3 from Molecular Probes for fixed cells.
 It can be excited with red lasers (633 nm works wery well and 647 can be used also),and its emission is in
the far red. It works very well for identified nucleus, but no for cell
cycle analysis. You can try also other dyes from molecular probes, in the
range of syto series.

Susanna Castel
Microscopia Confocal
SCT-UB


On Thu, 4 Oct 2001, 'Hesam' 'Dehghani' wrote:

> Dear list members,
>
> We have used GFPuv (which gets excited and emits at UV range) to tag proteins
> in yeast. But, now we are faced with the problem of DNA staining, since DAPI
> gets excited at the same range. Does any body know of any other kind of DNA
> stain in red range for yeast cells that is cell-membrane permeant? Or, any
> other way to show nuclei,e.g. a nuclear envelop stain in red range. Thank you.
>
> Hesam Dehghani
> Department of Chmistry and Biochemistry
> University of Guelph
> Guelph, Ontario, Canada
>