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Steve,
Does Cobolt's 491nm laser offer user-adjustable power
or is it 100mW on/off?
Regards,
David Basiji, Ph.D.
President and CEO, Amnis Corporation
2505 Third Ave., Suite 210
Seattle, WA 98121
(206) 374-7165 direct
(206) 919-3342 mobile
(206) 576-6895 fax
www.amnis.com
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> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[log in to unmask]] On Behalf Of Steve Knight
> Sent: Tuesday, July 03, 2007 12:35 PM
> To: [log in to unmask]
> Subject: Re: solid state lasers
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Commercial interest!
>
> As far as I know the majority of 488nm solid state lasers are
> not technically DPSS lasers but frequency doubled diode
> lasers (either optically or electrically pumped).
>
> They do exhibit excellent beam quality but as far as I know
> there is some uncertainty in wavelength (+/-2nm) depending on
> the diode that is frequency doubled.
>
> Cobolt manufacture true DPSS lasers operating at 491nm up to
> 100mW where the wavelength variation from laser to laser is
> much narrower.
>
> Regards
>
> Steve Knight
>
> -----Original Message-----
> From: "Guy Cox" <[log in to unmask]>
> To: [log in to unmask]
> Sent: 03/07/07 11:11
> Subject: Re: solid state lasers
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> One needs to be clear that there are two types of
> solid-state laser. Diode lasers have typically a
> relatively (in laser terms) broad range of wavelengths
> and often a rather non-circular beam profile. This
> means that a clean-up filter can often be useful.
> They typically offer wavelengths between about 380nm
> and 480nm and then from about 640nm on down. There
> are great wavelengths there for CFP but not YFP.
>
> DPSS (diode pumped solid state lasers) are crystal
> lasers which are pumped by diode lasers. The ones
> which are useful to us are frequency-doubled to
> give emission between 488nm and 561nm. These give
> very sharp, defined wavelengths just like gas lasers.
>
> Both types have great advantages over gas lasers in
> terms of heat load, avoidance of vibration, and at
> least in principle in life.
>
> Guy
>
>
> > Dear Marco,
> >
> > main advantages of dpss lasers are the small size, less
> heat emission and
> > usually longer lifetime and reasonable power output. On the
> other hand,
> > they are more sensitive to temperature and humidity fluctuations and
> > partly more difficult to couple. And there are still
> typical wavelengths
> > missing, like optimal excitation for CFP/YFP - but it
> depends on your
> > samples if this is an issue or not. If not, then I would
> recommend to go
> > for solid state.
> >
> > cheers,
> > Michael
> >
> >
> >> Search the CONFOCAL archive at
> >> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >>
> >> Dear list members
> >>
> >>
> >>
> >>
> >>
> >> We are in the market for a new confocal and I am currently
> examining
> >> differences in configurations, laser etc. Most companies
> are offering us
> >> systems with conventional Argon lasers and He/Ne. Olympus
> has indicated
> >> to
> >> me they would prefer to sell their system with solid-state
> lasers. They
> >> mention there is great advantage in price and durability
> and they are
> >> indeed
> >> better than conventional lasers. Could someone direct me to an
> >> independent
> >> source of information or please enlighten me whether this is indeed
> >> correct?
> >>
> >>
> >>
> >>
> >> Thanks
> >>
> >> Marco
> >>
> >>
> >>
> >> Dr. Marco Antonio Maximo Prado
> >>
> >> Departamento de Farmacologia, ICB, UFMG
> >>
> >> Av. Antonio carlos, 6627 31270-910
> >>
> >> Belo Horizonte, MG, Brasil
> >>
> >> tel 5531 34992718
> >>
> >> fax: 5531 34992695
> >>
> >> alternative e -mail: <mailto:[log in to unmask]> [log in to unmask]
> >>
> >> Cv Lattes <http://lattes.cnpq.br/8706664565242249>
> >> http://lattes.cnpq.br/8706664565242249
> >
>
>
> --
> Associate Professor Guy Cox
> Electron Microscope Unit,
> University of Sydney,
> NSW 2006, Australia
>
> Phone:+61 2 9351 3176 Fax:+61 2 9351 7682
> http://www.guycox.net
>
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