RNase digestion?
(1µg/ml RNase A in PBS, 30 min @ RT)
Nicely reduces cytosolic staining of PI.

-- 
Zsolt Lenkei, MD, PhD
Laboratoire de Neurobiologie
ESPCI - CNRS UMR 7637
10, rue Vauquelin
75005 Paris, France
Tel: (33-1) 40 79 51 84
Fax:  (33-1) 40 79 47 57



> From: Daniel Harrington <[log in to unmask]>
> Reply-To: Confocal Microscopy List <[log in to unmask]>
> Newsgroups: bit.listserv.confocal
> Date: Sat, 20 Jan 2001 10:48:26 -0600
> To: [log in to unmask]
> Subject: Re: Nuclear fluorochrome
> 
> Does anyone have any suggestions for reducing cytosolic staining with
> Sytox green?  I'm using it with 3T6 fibroblasts, and find that it
> stains the nucleus very nicely, but that there's still a fair bit of
> indiscriminate staining in the remainder of the cell body.  Any tips?
> 
> --Dan.
> 
>> Hi Tony,
>> 
>> Try Sytox Green for fixed cells (exc 488 , green emission). For live
>> cells have a look of all Syto- carbocyanines. All of them are from
>> Molecular Probes. They work well if you use at low concentrations.
> 
> _____________________________________________________________
> Daniel Harrington
> E-mail: [log in to unmask]
> Northwestern University
> Department of Materials Science and Engineering
> 2225 N. Campus Dr
> Evanston, IL 60208-3108
> 
> Office phone: 847-467-6431            Office: MLSB 3044
> Lab phone: 847-467-6417               Lab: MLSB 3032
> 847-467-6416                    MLSB 3026
>