CONFOCALMICROSCOPY Archives

September 1993

CONFOCALMICROSCOPY@LISTS.UMN.EDU
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Subject:
Re: dual labelling
From:
Alan Hibbs <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 22 Sep 1993 10:11:36 LCL
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text/plain (16 lines) 
In reply to the question by Stamatis as to why the laser will bleach only
one of the two dyes:
 
If you use a single line (488nm on an Argon ion laser) then the Texas Red
channel is being excited well outside its optimum wavelength and so is much
less sensitive to fading compared FITC.
 
bye, Alan.
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Dr. Alan R. Hibbs                              FAX: 07 362 0104
Queensland Institute of Medical Research     Phone: 07 362 0416
PO Royal Brisbane Hospital                   email: [log in to unmask]
Herston, Brisbane, Qld., 4029
AUSTRALIA
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