CONFOCALMICROSCOPY Archives

January 2004

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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From:
Martin Wessendorf <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 14 Jan 2004 13:06:25 -0600
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Michal Opas wrote:

> The problem consist in getting not-so-well-focused images on screen,
> while the cell viewed by eye appears super sharp.  We tested it on
> phalloidin-labelled cells: we see sharp stress fibres and striations in
> cardiomyocytes and the same stress fibres appear slightly blurred on the
> monitor screen and striations are hard to make up.

Is there any chance that it's just vibration?

Cables can sometimes transmit vibration to the table.  Also, we've had
trouble from time to time with one of the valves on our air-table
sticking in the "on" position so that the table is stuck at the top of
its excursion.  That ends up being little better than if the air were
off entirely.

Good luck!
--
Martin Wessendorf, Ph.D.                   office: (612) 626-0145
Assoc Prof, Dept Neuroscience                 lab: (612) 624-2991
University of Minnesota             Preferred FAX: (612) 624-8118
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: (612) 626-5009
Minneapolis, MN  55455                E-mail: [log in to unmask]

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