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Hi Kuyu:

You may be able to estimate the refractive index with the following formula:

n=n 1 V 1 +n 2 V 2 / V 1 +V 2 

n is the refractive index of the mixtrure, n1, n2 are that of the respective solution, and V1, V2 are their respective volume. We used this method to get the right refractive index at 80 degrees. 

De
________________________________________
From: Kuyu Wang [[log in to unmask]]
Sent: Monday, August 27, 2012 1:06 AM
To: [log in to unmask]
Subject: need to measure the refractive index of immersion oil

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Hi All:



I have a question about the refractive index of immersion oil. In my
experiment, I need to use the immersion oil as a control specimen to measure
the refractive index of other tissues. Here the immersion oil was slightly
stained for fluorescence. As the dye I used was very little amount, I
assumed that it would not alter the refractive index of the immersion oil.
However, this assumption must be verified as it is important to the accuracy
of the results.



With this background, I have two questions:



1. Does anybody have the similar experience? If yes, could you briefly
describe the situations you ever met?



2. If I want to experimentally measure the refractive index of the stained
immersion oil, what kind of instrument is proper to this work? I'm not
familiar with this kind of techniques and was completely confused after lots
of searching online.



Any suggestions or information will be appreciated.



All the best



Kuyu