Dear Tom,

we have generated two antibodies so far and both of them work at the western
blot as well as at the LM immunochemistry.  we are planning to check them
for the Immuno-EM soon.

By the way I would be happy if some one could provide me a protocol for
Immuno EM staining of ultrathin Drosophila tissue sections made using
LRwhite embeding media. We do not have a cryo-device here.

lots of thanks in advance,

krishanu.


----- Original Message -----
From: Tom Phillips <[log in to unmask]>
Newsgroups: bit.listserv.confocal
To: <[log in to unmask]>
Sent: Tuesday, September 19, 2000 12:16 AM
Subject: % of antibodies that work in immuno


> Lots of antibodies don't work at LM immunocytochemistry and even more
> don't work at the EM level.  I would like to make an informal survey.
> If you respond to me or the list, I will post a summary to the list.
>
> Here are the questions:
>
> Have you generated antibodies that work in some method (ELISA,
> Westerns, immunoprecipitation) but not LM immunocytochemistry?
>
> Were they monoclonals or polyclonals?
>
> What percentage of the antibodies you have made work at the LM level
> but not for EM immunocytochemistry?
>
> If you have made antibodies against synthetic peptides, what
> percentage work in either LM or EM?
>
> Thanks, Tom
>
> --
> Thomas E. Phillips, Ph.D.
> Associate Professor of Biological Sciences
> Director, Molecular Cytology Core Facility
>
> 3 Tucker Hall
> Division of Biological Sciences
> University of Missouri
> Columbia, MO 65211-7400
> (573)-882-4712 (voice)
> (573)-882-0123 (fax)