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Hi, Jelle. On the plus side, you got a hyperthermia device at no extra charge!
Seriously, though, I agree with Ben that your best bet may be to just unplug it or I might go one step further and remove the Super Galvo Z-stage completely. This stage looks great on paper but has some pretty serious drawbacks. We were quoted the galvo stage for an upright multiphoton system, but a mouse is too heavy for this thing (especially with any kind of heating pad to keep the mouse warm). This was offered as an alternative to a piezo objective positioner that we had asked for in the bid, but we didn't appreciate this key limitation of the weight until it was too late, so now it sits unused in a drawer. We also received 2 of these galvo stages on inverted microscopes. On a workhorse confocal (used by many users mostly for fixed cells) we have removed it because it was too easily damaged (replaced twice in the first 6 months or so) and doesn't accommodate easy switching of stage inserts (you have to fiddle with tiny little screws). And it wasn't really necessary - the built-in focus motor is sufficient for routine applications. On the other microscope we are still using it, but it's a bit of a pain and I wish we had a piezo instead. Tokai Hit has a special version of their stage-top incubator that is light enough and fits in the galvo stage, but if you add too much culture medium the weight is too great and the stage starts to squeal. We haven't noticed hyperthermia but maybe with the galvo stage exposed to the room and just a tiny stage-top incubator the passive heat dissipation is sufficient - but I'll have to double check the temperature in the coverglass chamber itself now that you've mentioned it. Good luck and let us know how you solve it!
Cheers,
James
-----------------------------------------------
James Jonkman, Staff Scientist
Advanced Optical Microscopy Facility (AOMF)
and Wright Cell Imaging Facility (WCIF)
University Health Network
MaRS, PMCRT tower, 101 College St., Room 15-305
Toronto, ON, CANADA M5G 1L7
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www.aomf.ca
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Benjamin Smith
Sent: Thursday, March 18, 2021 12:43 PM
To: [log in to unmask]
Subject: [External] Re: Heat production on SP8 super Z stage
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If you put galvo stages under load, they will heat-up because they are using constant current to maintain position. Perhaps the simplest solution would be to unplug the galvo when using the incubator and use the motorized stage/objective turret for XYZ scans (of course, this won't work for XZ scans).
This is exactly what we did when using a Peltier stage, which is relatively heavy for a galvo stage. Speaking of which, Peltier stages are perfect for both heating and cooling a sample very precisely. We used this stage for everything from animal cell culture (37.0 °C) to lamprey embryos (18.0 °C) and even used it to keep drosophila pupae at exactly 21.0 °C during a 48-hour live imaging run, to counteract both the temperature swings in the room and the gradual heating due to the microscope electronics. This is the Peltier stage we used: https://urldefense.com/v3/__https://physitemp.com/heatcoolstagesmicro_p216__;!!CjcC7IQ!erczknp8X8aXqIu62W0z5PW6sIGafkGHw5hd7oDysrStbX4OaBD3RGd89QqiP50oaxDj$ [physitemp[.]com]
Cheers,
Ben Smith
On Thu, Mar 18, 2021 at 3:55 AM Jelle Postma <[log in to unmask]> wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
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> lmicroscopy__;!!CjcC7IQ!erczknp8X8aXqIu62W0z5PW6sIGafkGHw5hd7oDysrStbX4OaBD3RGd89QqiP-FO2t5M$ [lists[.]umn[.]edu] Post images on https://urldefense.com/v3/__http://www.imgur.com__;!!CjcC7IQ!erczknp8X8aXqIu62W0z5PW6sIGafkGHw5hd7oDysrStbX4OaBD3RGd89QqiPwbBCSqh$ [imgur[.]com] and include the link in your posting.
> *****
>
> Dear all,
>
> I’m very thankful this list exists and have been reading along with
> great interest! I’d like to discuss a problem that we experience on
> one of our systems as follows:
>
> - We have a Leica SP8 with The Box/The Cube temperature controlled
> incubator, Super-Z galvo stage, and CO2/Humidity controlled lid to
> cover the sample area.
>
> - We notice that the super-z galvo stage becomes noticeably warm to
> the touch when in use, which affects the samples from our users, who
> often perform long-term imaging of live plants in petri dishes.
>
> - The local heat at the stage leads to condensation in the sample
> dishes, drying-out of growing media, and consequentially, unreliable
> results in the long term imaging experiments.
>
> - Unfortunately, The Box/The Cube cannot counteract the heat
> development, as the incubator environment is kept at the correct
> temperature, but the stage itself strongly deviates upward.
>
> So far, we are able to *slightly* alleviate the problem via bottles of
> ice water, but this is highly variable and short-term. Sub-optimal...
>
> As far as I am aware, there are no super-Z compatible sample-area
> coolers (but plenty of heaters), and the closest Leica is offering is
> an objective turret cooler (close, but not the right part)… I am
> wondering if there are obvious solutions to this that I am missing,
> whether some of you have found the same problem and possibly an
> impromptu solution, or whether there are aftermarket parts that counteract this issue.
>
> Thank you very much for your thoughts!
>
> ——————
>
> Dr. Jelle Postma
> Light microscopy and image analysis
> General Instrumentation, FNWI
> Radboud University Nijmegen
> Huygens Building, Room HG.01.222
> Phone: 0031 24 36 52199
>
--
Benjamin E. Smith, Ph. D.
Imaging Specialist, Vision Science
University of California, Berkeley
195 Weill Hall
Berkeley, CA 94720-3200
Tel (510) 642-9712
Fax (510) 643-6791
e-mail: [log in to unmask]
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