Hi: I can't justify neuro...I work on plants. I may well go to Cell
Bio...not sure at this point.But I have two daughters and a granddaughter
in sF so it is tempting.
We placed the specimen between two coverglasses. The bigger coverglass was
scotchtaped to the holder. specimen put in place, and coverglass on top
...usually used valap to seal it. The lower coverglass can be treated as
the slide.
If you come this way ...call me. Nina
>Hi!!!!
>
>Glad to hear from you.
>
>The second part of Julie's question threw me for a loop: did you put
>something on the slide to keep the spacer from leaking or was capillary
>action sufficient? I couldn't remember (it's only been the better part of
>a decade!).
>
>How are things going for you? What are you working on?
>
>Ironically, I was in Raleigh earlier this year on business. I think I knew
>that you were at NC State at that time and had a fleeting thought of giving
>you a call. As it was, we ended up "on assignment" from about 6:30AM until
>about 9:30 or 10:00 each night.
>
>Will you be at either Cell or Neuro? I understand that Neuro is becoming
>quite a haven for digital imaging.
>
>TTYS.
>Barbara
>
>
>
>At 05:37 PM 9/23/98 +0100, you wrote:
>>barbara: YES,I am listening. I am at NC State University (not Chapel
>>Hill)... I still have those aluminum holders and use them when we have
>>larger cells and are limited in distance between the condenser and
>>objective lens. They were made for me at the Marine Biological Laboratory
>>at their machine shop and I would imagine they still have the template. We
>>used to give one to every student in the course. Call 508-548-3705 and
>>ask for the machine shop.
>>Or just call me for information. Cheers, Nina
>>>Pat,
>>>
>>>Nina Allen (Wade-Forest, now at NCU or NC State) made some great spacers
>>>>from aluminum. I first saw them some years ago at her course at Woods
>>>Hole. They were cut about 1" x 2", with a channel in the middle for your
>>>sample (essentially a squarish "U") and, as I remember, were used with the
>>>large, rectangular coverslips
>>>
>>>Nina, if you are listening, please respond.
>>>
>>>Hope this is helpful,
>>>Barbara Foster
>>>Consortium President
>>>Microscopy/Microscopy Education
>>>Training solutions for improved productivity
>>>
>>>125 Paridon Street Suite 102
>>>Springfield, MA 01118
>>>PH: (413)746-6931 FX: (413)746-9311 email: [log in to unmask]
>>>Visit our web site: <http://www.MME-Microscopy.com/education>
>>>******************************************************
>>>MME is America's first national consortium dedicated to
>>>customized on-site training in all areas of
>>>microscopy, sample preparation, and image analysis.
>>>
>>>
>>>
>>>
>>>At 11:29 AM 9/23/98 -0500, Carol Bayles wrote:
>>>>Pat,
>>>>I use a very simple method. I break up coverslips and use them as spacers.
>>>>They are very uniform and come in varying thicknesses. You can always
>>>>double them if necessary. Just be careful not to cut yourself!
>>>>
>>>>Carol
>>>>
>>>>>I am trying to stain whole heart valve leaflets, with some
>>>>>success. However, mounting the end result in order to view, is
>>>>>proving difficult. Each leaflet is of variable thickness i.e. it
>>>>>has an undulating surface. Does anyone have any suggestions? I
>>>>>have tried making supports on a glass slide with mounting fluid or
>>>>>nail varnish, but it is difficult to end up with a uniform depth.
>>>>>Thanks in advance,
>>>>>Pat Taylor.
>>>>
>>>>
>>>>Carol Bayles 607-254-4860
>>>>Microscopy and Imaging Facility 607-254-4847 fax
>>>>BioResource Center [log in to unmask]
>>>>160 Biotech Bldg
>>>>Cornell University
>>>>Ithaca NY 14850
>>>>
>>>>
>>
>>Nina Stromgren Allen
>>Professor, Department of Botany
>>Box 7612
>>North Carolina State University
>>Raleigh, NC 27695-7612
>>Phone: 919-515-8382 (Office), 515-3525 (Lab), 515-2727 (Department
>Secretary)
>>Fax: 919-515-3436
>>
>>
Nina Stromgren Allen
Professor, Department of Botany
Box 7612
North Carolina State University
Raleigh, NC 27695-7612
Phone: 919-515-8382 (Office), 515-3525 (Lab), 515-2727 (Department Secretary)
Fax: 919-515-3436
|
