Dear Renato, and the list.
Yes, I enjoyed gorgeous autumn weather over the weekend!
While I am not in a position to officially represent Yokogawa, as Renato has mentioned already, official comment from Yokogawa, if requested, will be to ask to use the CSU spinning disk confocal system within 400nm-700nm wavelength range for best performance with a good reason.
As Lisa kindly advised, it is quite OK to use the CSUX1 system at 405 nm.
So, if your are talking about 405nm, it is within our specification range.
However, if you are thinking about using 351/363 nm, as is well understood, imaging at UV range below 400nm could be very difficult, which is not unique to the imaging with the CSU system but universal to all optical and/or imaging devices.
We have well confirmed the performance of the CSU confocal system including all components such as the microscope, laser and fibers, all filters, detector, etc.at 400-700 nm range.
However, we have no conclusive data on the use below 400nm range, in reality.
Our engineers don't think there could be any simple and easy way to optimize the CSU head for imaging at UV range, since every piece of optical parts both inside and outside of the CSU head requires optimization for UV.
It may be possible to capture images below 400nm, but, we cannot guarantee good-enough light efficiency, confocality or chromatic aberration correction, and thus cannot endorse it.
I am sorry for not being able to offer any quick solution but please kindly understand there is no magic in good UV imaging.
Mizuho Shimizu
Yokogawa Electric Corporation
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Renato Mortara
Sent: Monday, November 16, 2009 7:06 PM
To: [log in to unmask]
Subject: RES: Yokogawa lenses
Mizuho
I hope you had a terrrific and relaxing weekend !
Cheers
Renato
-----Mensagem original-----
De: Confocal Microscopy List [mailto:[log in to unmask]] Em nome de [log in to unmask] Enviada em: sábado, 14 de novembro de 2009 21:14
Para: [log in to unmask]
Assunto: Re: Yokogawa lenses
Hi Alby, Guy, Renato and the list,
Please allow us to "enjoy" a weekend holiday before getting back with information to answer some of your questions regarding Yokogawa spinning disk.
I wish everybody to have a nice holiday, too！
Cheers,
Mizuho
***********************************************************
Mizuho Shimizu
International Sales Team
BIO Group
B&A Center
Measurement Business Headquarters
Yokogawa Electric Corporation
2-3 Hokuyodai, Kanazawa-shi, Ishikawa
920-0177 JAPAN
Phone: +81-76-258-7028 Fax: +81-76-258-7029 <http://www.yokogawa.com/scanner/index.htm>
***********************************************************
________________________________________
差出人: Confocal Microscopy List [[log in to unmask]] は
Renato A. Mortara [[log in to unmask]] の代理
送信日時: 2009年11月15日 5:46
宛先: [log in to unmask]
件名: Re: Yokogawa lenses
Alby and Guy,
sure enough heating with UV is a concern.
I did wish someone from Yokogawa read this list and gave a conclusive answer whether any non UV-compatible element in the lightpath could be customized to be UV friendly.
Grazie and Ciao,
Renato
Renato A. Mortara
Disciplina de Parasitologia
UNIFESP Escola Paulista de Medicina
R. Botucatu, 862 6o andar
04023-062
S?o Paulo SP
Brasil
Quoting Alberto Diaspro <[log in to unmask]>:
> It is possible that they also have to take into account the possible
> heating due to intense UV ciao Alby
>
>
>
>
> ISTITUTO ITALIANO
> DI TECNOLOGIA
>
> Prof. Alberto Diaspro
> Scientific Head
> Nanophysics
> Via Morego, 30 16163 Genova
> Tel: +39-010.71.781.503
> Fax +39-010-72.03.21
> Mobile +39-3666719968
> www.iit.it
>
>
> On Nov 14, 2009, at 12:55 PM, Guy Cox wrote:
>
>> OK, from another commercial person (who does not want to be named)
>> I've now heard that in fact the Yokogawa microlenses are in fact
>> silica (as I had suspected). But the snag is that there is another
>> optic in the system which does not pass UV. This is a bit more
>> promising, since replacing the microlenses is not an option but
>> replacing a transfer lens shouldn't be difficult. Someone from
>> Yokogawa must surely be reading this list. One assumes, also, that
>> they would be interested in extending the application range of their
>> system. So, come on, Yokogawa, please give us the real story!
>>
>> Guy
>>
>>
>> Optical Imaging Techniques in Cell Biology
>> by Guy Cox CRC Press / Taylor & Francis
>> http://www.guycox.com/optical.htm
>> ______________________________________________
>> Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope
>> Unit, Madsen Building F09, University of Sydney, NSW 2006
>> ______________________________________________
>> Phone +61 2 9351 3176 Fax +61 2 9351 7682
>> Mobile 0413 281 861
>> ______________________________________________
>> http://www.guycox.net
>>
>>
>>
>
> ----------------------------------------
> Alberto Diaspro
> Head, Nanophysics Unit
> Senior Scientist
> The Italian Institute of Technology -IIT Via Morego, 30
> 16163 - Genova (Italy)
> phone: +39 010 71781503
> mobile: +393666719968
> fax: +39 010 720321
> http://www.iit.it
> [log in to unmask]
>
> Professor of Applied Physics
> Department of Physics
> University of Genova
> Via Dodecaneso, 33
> 16146 Genova - Italy
> tel. +39 010 353 6426
> fax. +39 010 314218
> http://www.lambs.it
> [log in to unmask]
> -------------------------------------------------------
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