Hi Richard and list members,
It is great that you will be able to bench test UV lines on the Yokogawa head and fibers.
Keeping my fingers crossed for a positive outcome !
Best
Renato
-----Mensagem original-----
De: Confocal Microscopy List [mailto:[log in to unmask]] Em nome de Richard Berman
Enviada em: terça-feira, 17 de novembro de 2009 20:32
Para: [log in to unmask]
Assunto: Re: RES: Yokogawa lenses
******** commercial interest *************
Hi Renato and Confocal List: As I stated in a previous post, we will be investigating the potential to operate the CSU at 375nm shortly. We expect the laser in about 2 weeks. As has been mentioned by another member, the potential to damage optics must always be considered. We do not believe that damage due to heating will be a factor since the heat is distributed over a fairly large area of the spinning disk. In fact if there is an issue it will be with the pinhole disk where the power densities are much higher. Damage due to UV is a greater concern but can be assessed. Many higher quality lenslet arrays are made from fused silica. Transmission tests will enable us to determine if either of the disks are a polymer or both are fused silica. If both disks are silica they are probably not UV grade fused silica, but since we are not going to hard UV the standard grade will almost certainly show no degradation at 375nm. And by the way, single mode fibers that are rated to work at 375nm are made from the same fused silica as fibers for longer wavelengths. The power densities in the fibers are orders of magnitude higher than what will be hitting the disks and so degradation will be evident in the fiber long before the disks. This is something we are going to be assessing more carefully moving forward and should not be considered a guarantee of performance at this time.
And on a slightly different note, we have been very successful modifying the CSU to use lasers above 700nm, with emission wavelengths approaching 800nm. We have given the modifications a commercial name, BOREALIS. We are very excited about this development and our partner Quorum Technologies (also a commercial interest) has successfully installed two such systems with great results. Just to say that moving beyond the standard 400nm - 700nm range is possible.
Best Regards, Richard
Renato Mortara wrote:
> What is great in this list is that all the information flows along
> opinions and ideas. I agree with Guy as well as Paul. Unfortunately, I
> do not have enough $$ to play around with the systems,fibers,etc, just
> to try to implement an application... - my 2 cents in Brazilian Reais,
> ± $0.10
>
> Best
>
> Renato
>
> -----Mensagem original-----
> De: Confocal Microscopy List [mailto:[log in to unmask]]
> Em nome de Paul Maddox Enviada em: terça-feira, 17 de novembro de 2009
> 14:54
> Para: [log in to unmask]
> Assunto: Re: Yokogawa lenses
>
> Part of the "problem" here might be in way the question is being
> asked. In my experience, the "end users" should take the initiative
> to experiment and try new applications of existing equipment. Thereby
> we can empirically test these ideas on real world experimental
> applications. However, to properly execute this, we need to know
> certain things such as if UV light (eg
> 300-400nm) will damage the system. The question of if it will work or not is up to us to determine (ultimately by peer review). The proper question to Yokogawa in this case is will we damage the system in doing so?
>
> Clearly, the greater the exchange of information the better for all involved. But in practical terms, there is never a 100% free flow of info and I doubt there will be in the very near future.
>
> These are my two cents (Canadian, so ±1.8 US)...
>
> Paul
>
> Paul S. Maddox, PhD
> Assistant Professor
> Institute for Research in Immunology and Cancer Dept of Pathology and
> Cell Biol, U. de Montreal P.O. Box 6128, Station Centre-Ville Montréal
> QC H3C 3J7 CANADA
>
> Courier:
> 2900, boulevard Édouard-Montpetit
> Pavillon Marcelle-Coutu, Quai 20
> Montreal QC H3T 1J4
> CANADA
>
> [log in to unmask]
> Ph: 514-343-7894
> Fax: 514-343-6843
>
>
>
>
> On 17/11/09 11:17 AM, "Neeraj Gohad" <[log in to unmask]> wrote:
>
>
>> I agree with Guy, there is much to be accomplished, the companies
>> should stopped treating scientist as just end users and we should
>> stop treating them as just vendors. Both parties will definitely
>> benefit if there is real dialogue.
>>
>> Best,
>>
>> Neeraj.
>>
>>
>> Neeraj V. Gohad, Ph.D.
>> Postdoctoral Fellow
>> Okeanos Research Group
>> Department of Biological Sciences
>> 132 Long Hall
>> Clemson University
>>
>> Please note my new email address: [log in to unmask]
>>
>>
>>
>>
>> -----Original Message-----
>> From: Confocal Microscopy List
>> [mailto:[log in to unmask]] On Behalf Of Guy Cox
>> Sent: Tuesday, November 17, 2009 6:18 AM
>> To: [log in to unmask]
>> Subject: Re: Yokogawa lenses
>>
>> Well, I'm glad your weekend was good (so was mine actually, with
>> kangaroos all around and lyre-birds playing in the garden).
>>
>> But your considered opinion after all that was to give us NO
>> information. We are scientists, we are a community. The
>> relationship between manufacturers and users is what drives advances in the microscopy field. Please be a player
>> in this - it really is to your advantage. What we need to know is simple -
>> something you can tell us in an instant. What is the wavelength
>> transmission range of your micro-lenses? For what wavelength are
>> they chromatically corrected? What other components in your head
>> might affect the wavelength transmission?
>>
>> These are not great secrets of huge commercial value. In fact,
>> someone will certainly make the relevant measurements and post them
>> if you don't. But in the end collaboration is what drives progress,
>> so I hope you will change your mind and decide to become part of the community.
>>
>>
>>
>> Guy Cox
>>
>>
>>
>> Optical Imaging Techniques in Cell Biology
>> by Guy Cox CRC Press / Taylor & Francis
>> http://www.guycox.com/optical.htm
>> ______________________________________________
>> Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope
>> Unit, Madsen Building F09, University of Sydney, NSW 2006
>> ______________________________________________
>> Phone +61 2 9351 3176 Fax +61 2 9351 7682
>> Mobile 0413 281 861
>> ______________________________________________
>> http://www.guycox.net
>> -----Original Message-----
>> From: Confocal Microscopy List
>> [mailto:[log in to unmask]] On Behalf Of
>> [log in to unmask]
>> Sent: Tuesday, 17 November 2009 12:20 PM
>> To: [log in to unmask]
>> Subject: Re: Yokogawa lenses
>>
>> Dear Renato, and the list.
>>
>> Yes, I enjoyed gorgeous autumn weather over the weekend!
>>
>>
>> While I am not in a position to officially represent Yokogawa, as
>> Renato has mentioned already, official comment from Yokogawa, if
>> requested, will be to ask to use the CSU spinning disk confocal
>> system within 400nm-700nm wavelength range for best performance with a good reason.
>>
>> As Lisa kindly advised, it is quite OK to use the CSUX1 system at 405 nm.
>> So, if your are talking about 405nm, it is within our specification range.
>>
>> However, if you are thinking about using 351/363 nm, as is well
>> understood, imaging at UV range below 400nm could be very difficult,
>> which is not unique to the imaging with the CSU system but universal
>> to all optical and/or imaging devices.
>> We have well confirmed the performance of the CSU confocal system
>> including all components such as the microscope, laser and fibers,
>> all filters, detector, etc.at 400-700 nm range.
>> However, we have no conclusive data on the use below 400nm range, in reality.
>>
>> Our engineers don't think there could be any simple and easy way to
>> optimize the CSU head for imaging at UV range, since every piece of
>> optical parts both inside and outside of the CSU head requires optimization for UV.
>> It may be possible to capture images below 400nm, but, we cannot
>> guarantee good-enough light efficiency, confocality or chromatic
>> aberration correction, and thus cannot endorse it.
>>
>> I am sorry for not being able to offer any quick solution but please
>> kindly understand there is no magic in good UV imaging.
>>
>>
>> Mizuho Shimizu
>>
>> Yokogawa Electric Corporation
>>
>>
>>
>>
>> -----Original Message-----
>> From: Confocal Microscopy List
>> [mailto:[log in to unmask]] On Behalf Of Renato Mortara
>> Sent: Monday, November 16, 2009 7:06 PM
>> To: [log in to unmask]
>> Subject: RES: Yokogawa lenses
>>
>> Mizuho
>> I hope you had a terrrific and relaxing weekend !
>>
>> Cheers
>>
>> Renato
>>
>> -----Mensagem original-----
>> De: Confocal Microscopy List
>> [mailto:[log in to unmask]] Em nome de
>> [log in to unmask] Enviada em: sábado, 14 de novembro de
>> 2009
>> 21:14
>> Para: [log in to unmask]
>> Assunto: Re: Yokogawa lenses
>>
>> Hi Alby, Guy, Renato and the list,
>>
>> Please allow us to "enjoy" a weekend holiday before getting back with
>> information to answer some of your questions regarding Yokogawa spinning disk.
>>
>> I wish everybody to have a nice holiday, too！
>>
>> Cheers,
>>
>> Mizuho
>>
>> ***********************************************************
>> Mizuho Shimizu
>> International Sales Team
>> BIO Group
>> B&A Center
>> Measurement Business Headquarters
>> Yokogawa Electric Corporation
>> 2-3 Hokuyodai, Kanazawa-shi, Ishikawa
>> 920-0177 JAPAN
>> Phone: +81-76-258-7028 Fax: +81-76-258-7029
>> <http://www.yokogawa.com/scanner/index.htm>
>> ***********************************************************
>> ________________________________________
>> 差出人: Confocal Microscopy List [[log in to unmask]] は
>> Renato A. Mortara [[log in to unmask]] の代理
>> 送信日時: 2009年11月15日 5:46
>> 宛先: [log in to unmask]
>> 件名: Re: Yokogawa lenses
>>
>> Alby and Guy,
>>
>> sure enough heating with UV is a concern.
>>
>> I did wish someone from Yokogawa read this list and gave a conclusive
>> answer whether any non UV-compatible element in the lightpath could
>> be customized to be UV friendly.
>>
>> Grazie and Ciao,
>>
>> Renato
>>
>>
>> Renato A. Mortara
>> Disciplina de Parasitologia
>> UNIFESP Escola Paulista de Medicina
>> R. Botucatu, 862 6o andar
>> 04023-062
>> S?o Paulo SP
>> Brasil
>>
>>
>> Quoting Alberto Diaspro <[log in to unmask]>:
>>
>>
>>> It is possible that they also have to take into account the possible
>>> heating due to intense UV ciao Alby
>>>
>>>
>>>
>>>
>>> ISTITUTO ITALIANO
>>> DI TECNOLOGIA
>>>
>>> Prof. Alberto Diaspro
>>> Scientific Head
>>> Nanophysics
>>> Via Morego, 30 16163 Genova
>>> Tel: +39-010.71.781.503
>>> Fax +39-010-72.03.21
>>> Mobile +39-3666719968
>>> www.iit.it
>>>
>>>
>>> On Nov 14, 2009, at 12:55 PM, Guy Cox wrote:
>>>
>>>
>>>> OK, from another commercial person (who does not want to be named)
>>>> I've now heard that in fact the Yokogawa microlenses are in fact
>>>> silica (as I had suspected). But the snag is that there is another
>>>> optic in the system which does not pass UV. This is a bit more
>>>> promising, since replacing the microlenses is not an option but
>>>> replacing a transfer lens shouldn't be difficult. Someone from
>>>> Yokogawa must surely be reading this list. One assumes, also, that
>>>> they would be interested in extending the application range of
>>>> their system. So, come on, Yokogawa, please give us the real story!
>>>>
>>>> Guy
>>>>
>>>>
>>>> Optical Imaging Techniques in Cell Biology
>>>> by Guy Cox CRC Press / Taylor & Francis
>>>> http://www.guycox.com/optical.htm
>>>> ______________________________________________
>>>> Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope
>>>> Unit, Madsen Building F09, University of Sydney, NSW 2006
>>>> ______________________________________________
>>>> Phone +61 2 9351 3176 Fax +61 2 9351 7682
>>>> Mobile 0413 281 861
>>>> ______________________________________________
>>>> http://www.guycox.net
>>>>
>>>>
>>>>
>>>>
>>> ----------------------------------------
>>> Alberto Diaspro
>>> Head, Nanophysics Unit
>>> Senior Scientist
>>> The Italian Institute of Technology -IIT Via Morego, 30
>>> 16163 - Genova (Italy)
>>> phone: +39 010 71781503
>>> mobile: +393666719968
>>> fax: +39 010 720321
>>> http://www.iit.it
>>> [log in to unmask]
>>>
>>> Professor of Applied Physics
>>> Department of Physics
>>> University of Genova
>>> Via Dodecaneso, 33
>>> 16146 Genova - Italy
>>> tel. +39 010 353 6426
>>> fax. +39 010 314218
>>> http://www.lambs.it
>>> [log in to unmask]
>>> -------------------------------------------------------
>>>
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>
>
--
Richard Berman
Spectral Applied Research
9078 Leslie St., Unit 11
Richmond Hill, Ontario
L4B 3L8
905-326-5040 ext. 444
www.spectral.ca
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