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With the 80/20 filter 80% of the light is transmitted and 20% is
reflected. The Meta detector is designed to fysically block the laser
lines before they enter the detectors, so the reflection of the laser
shouldn't interfere. What you will see in your spectrum is small dips at
the laserlines (also the non-active lasers). It's important to keep the
pinhole at 1 airy unit. In practice the blocking of the laser lines is
not always perfect. However when it's very bad you should contact your
service engineer to improve the performance.

Regards,
Gert van Cappellen

Tamara Casavan wrote:

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>We are using a neutral density filter NT 80/20 on the Zeiss LSM 510 META
>system.  Does anyone know what the 80/20 means?  Also, if we use this dichroic
>during a lambda scan and include the laser line in our detection range - will
>the laser damage the PMT's?
>
>Tami Casavan
>UND Medical School
>
>

--

wigGert van Cappellen
Reproduction and Development, Erasmus MC, Tel. +31-10-40 87578 FAX +31-10-40 89461
Dr. MOlenwaterplein 50, 3015 GE ROTTERDAM, Netherlands
mailto:[log in to unmask]
http://www.eur.nl/fgg/endov/multiphoton
applied Optical Imaging Centre
http://www.eur.nl/fgg/molmed/OIC/