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March 2008

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Subject:
hPa injection pressure and volume calculation
From:
Vasseur Monique <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 28 Mar 2008 10:02:18 -0400
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Dear all,

We are using an Eppendorf FemtoJet to inject femtoliters of solution in living cells.  The instruction manual says you multiply the pressure by the injection time to get the volume injected...  Pressure is in hPa and time in second.  I don't have any idea to what hPa corresponds and what should I get as a result: microliters? femtoliters?  Is there other variable to put in the equation to get the volume (we use FemtoTips)?

Thanks a lot for your help.
 
Monique Vasseur
Microscopie et imagerie
Département de biochimie
Université de Montréal
C.P. 6128, succursale Centre-ville
Montréal QC    H3C 3J7   Canada
tél. (514) 343-6111 poste 5148

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