CONFOCALMICROSCOPY Archives

August 1992

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Subject:
Re: Zeiss LSCM
From:
Paul Goodwin <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Mon, 10 Aug 1992 07:39:48 -0700
Content-Type:
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On Mon, 10 Aug 1992, Jim Barrett wrote:
 
>
> Our z-step is about 180 nanometers, the pixel size or  x/y resolution
> is around 30 nanometers. In order to get a correct aspect ratio
> I thought I should multiply (via trilinear interpolation) the z
> slices by 6 (= 180/30).
>
> -Jim
 
Jim-
 
Do you really get a Z-res of 180nm and an x-y of 30 nm. I am impressed!
Did you mean that you would multiply the Z step by 6 or divide? It seems
to me that the z-step is 6x "too large" and should be reduced to 1/6.
 
 
Paul

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