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Hi Amtabha,

It is possible to see FM1-43 fluorescence after fixation. The major
problem is that if you use glutharaldehyde as fixative, it interferes with
FM1-43 fluorescence thus you need to incubate your prep with ammonium
clhoride before imaging. I did this procedure for photoconversion
purposes  at the frog neuromuscular junction and   this method  was described at
Neuron (2000) volume 27,551-559. Please do not hesitate to contact me directly
([log in to unmask]) if you have questions.
Good luck
Cristina

Cristina Guatimosim, Ph.D
Departamento de Farmacologia, ICB
UFMG
Av. Antonio Carlos 6627
Belo Horizonte, MG
Brazil

Amitabha Majumdar wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> I would like to know whether one can image FM1-43 dye uptake after
> fixation.If yes what type of fixation will help.I am trying to do the
> experiment on drosophila neuromascular junction by stimulating using high
> K.Does it bleaches very fast.
>
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>
>                   Amitabha Majumdar.
>                    Research Scholar
>             Department of Biological Sciences.
>           Tata Institute of Fundamental Research.
>                  Homi Bhaba Road,Colaba
>                     Mumbai-400005
>                        India.
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