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I have been using Helicon Focus for the past year and it works very well. Here is a link with a few examples. http://www.heliconsoft.com/heliconfocus.html

Cheers, Michael

Michael Weis
Electron Microscopy & Digital Imaging
Pacific Agri-Food Research Centre
Agriculture and Agri-Food Canada
4200 Highway 97
Summerland, BC  V0H 1Z0 
Telephone: 250-494-6410
Facsimile: 250-494-0755
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-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Carl Boswell
Sent: Friday, October 05, 2007 4:35 PM
To: [log in to unmask]
Subject: software


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Dea all,
Does anyone know of software that selects only in focus portions of an image 
and discards the out-of-focus parts.  We need to get clear views of a curved 
surface (the fly eye) with a 10x objective using reflected light.  Only thin 
optical slices are in focus at this mag, but if we could put together the 
portions that are in focus over the entire depth of the specimen, we could 
rebuild a clean image of the structure.  This wouild be a used in an assay 
screening a bizillion flies, so SEM is not really practical.
thanks,
Carl

Carl A. Boswell, Ph.D.
Molecular and Cellular Biology
University of Arizona
520-954-7053
FAX 520-621-3709