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Heyo,
On thing you could try would be to soak the paper in fluorescein, wash out the excess, and then mount the paper in water or glycerol (which is closer to the actual refractive index of cellulose - 1.469). Multi-photon will allow you look even further into the sample, if available.
As others suggested, a normally more expensive option, but one that would allow you to see through the full depth of the sample with equal resolution, would be to use a microCT.
Cheers,
Ben Smith
Benjamin E. Smith, Ph.D.
Samuel Roberts Noble Microscopy Laboratory
Research Scientist II
University of Oklahoma
Norman, OK 73019
E-mail: [log in to unmask]
Voice 405-325-4391
FAX 405-325-7619
http://www.microscopy.ou.edu/
________________________________________
From: Confocal Microscopy List [[log in to unmask]] on behalf of Dar Do [[log in to unmask]]
Sent: Tuesday, May 06, 2014 8:58 AM
To: [log in to unmask]
Subject: IMAGING OF CELLULOSE
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Hello,
Does anybody know of microscopy techniques for 3D imaging of paper or other
cellulose based fabrics? We have been trying with confocal microscopy and
did imaging of the surface of the outer layer of cellulose fibers, but were
unable to go through the fibers and image the underlying layers.
Best regards,
Dario
--
Dario Donnarumma
PhD student
personal blog <http://dariodonnarumma.blogspot.it/>
tel: +39 081 7682261
fax: +39 081 2391800
*Chemical Engineering @ the Micro-Scale (ChemEMS)*
*Dipartimento di Ingegneria Chimica, *
*dei Materiali e della Produzione Industriale (DICMAPI)*
*Università degli Studi di Napoli Federico II*
*Piazzale V. Tecchio 80 Napoli*
*I-80125 ITALIA*
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