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>You want to use coverslips that are 0.17 mm thick - number 1.5
>coverslips are the closest in thickness.

The trouble with No. 1.5 is that there is great variation in thickness. 
The industry standard for No. 1.50 is:
#1.5 = 0.160  -  0.190

Please review the achieves under Subject: "Coverslip - Good News'

This is quite a large range, and 0.190 mm thick coverslips will cause detrimental spherical aberration.
 
Both Menzel and Assistent (Hecht Glaswarenfabrik [Thanks Beat]) sell a better standard thickness for confocal microscopy it is called:
"170 +/- 0.01mm"
 
Many of the distributors may not know about this sorting of coverslip, but it does exist. You may need to convince them to ask their suppliers about it.
 
If you still have trouble you can order them from Lomb in Australia, they will deliver.

I order "0.170 +/- 0.01mm" coverslips for confocal through 
Lomb Scientific www.lomb.com.au . This is an Australian distributor 
of Menzel Glass. Lomb will also cut coverslips to special custom sizes 
(larger bulk orders are more economical). 

You can contact Peter directly on [log in to unmask]  for product
information, order codes etc..

Or as Peter suggests he is not always available [log in to unmask]  may
work better sometimes.

I have no commercial affiliation with any of the companies listed. I can prove it, have a look at the car I drive! :)
 
Cheers
Stephen H. Cody
Microscopy Manager
Central Resource for Advanced Microscopy
Ludwig Institute for Cancer Research
PO Box 2008 Royal Melbourne Hospital
Parkville, Victoria,      3050
Australia
Tel: 61 3 9341 3155    Fax: 61 3 9341 3104
email: [log in to unmask]
www.ludwig.edu.au/labs/confocal.html
www.ludwig.edu.au/confocal

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________________________________

From: Confocal Microscopy List on behalf of Kurt Thorn
Sent: Fri 26/10/2007 6:33 AM
To: [log in to unmask]
Subject: Re: coverslips for cell culture



Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

You want to use coverslips that are 0.17 mm thick - number 1.5
coverslips are the closest in thickness.

You can also use coverslip bottom plates or coverlsip bottom dishes -
these are very convenient if you want to image the cells live but may be
more of a pain for fixing and staining.  Glass-bottom-dishes.com sell a
number of different varieties.

Kurt

Fredrik Wermeling wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi!
>
> 
>
> I'm planning to grow adherent cells for later staining with
> fluorescently labelled antibodies. Previously I've done this by adding
> a sterile round coverslip in a 12 well plate and kept the cells on the
> coverslip through out the staining and fixation and then just
> "mounted" them on a microscope slide. My questions are:
>
> - Do you have any suggestions for which coverslips to purchase, I
> guess they have to be thin enough and without auto fluorescence.
>
> - Is there any alternative? I saw that Nunc sells a Lab tek product
> that sounds like what I´m looking for.
>
> 
>
> Thanks a lot
>
> / Fredrik
>
> 
>


--
Kurt Thorn, PhD
Director, Nikon Imaging Center
University of California San Francisco

UCSF MC 2140
Genentech Hall Room S252
600 16th St.
San Francisco, CA 94158-2517

http://nic.ucsf.edu <http://nic.ucsf.edu/> 
phone 415.514.9709
fax   415.514.4300