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June 2013

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Subject:
From:
Paul Herzmark <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 4 Jun 2013 21:45:14 -0700
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*****
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Drill a hole and glue on your cover slip. Easy!

Paul Herzmark


On Tue, Jun 4, 2013 at 7:10 PM, jerry sedgewick <[log in to unmask]>wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>
> *****
>
> Hello all,
>
> I'm doing a demonstration for live cell imaging using an inverted,
> fluorescence microscope.  I'd like to do a fairly low tech method for the
> first demo.  My thought is to use a plastic culture dish, cut a square in
> the bottom, and then glue a cover slip to the bottom.  I will be filling it
> with pond water since I should find plenty of autofluorescent organisms.
>
> I recall having tried to cut through a plastic culture dish and it wasn't
> easy.  I'm wondering if any of you have ever tried this and have had
> success at cutting through this sort of dish.  How did you do it while
> retaining smooth edges?
>
> Any other means for accomplishing the same end would be appreciated for a
> project without a budget.
>
> Thanks!
>
> Jerry Sedgewick
>

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