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Hi everyone,

Would anyone be able to point me to research papers, products, or any other types of information on building or purchasing a stage-top microscope incubator for live cell imaging with somewhat unusual requirements? The incubator should have two glass windows on either side to support transillumination imaging with a long working distance air objective. The microscope will be oriented horizontally, and so the two windows should lie in the vertical plane. The cell buffer should be maintained at physiological temperatures (between 30 and 37 deg. C). A CO2 environment is not necessary as we expect the cell sample to be completely submerged between the two windows in HEPES buffer for at most ~10 minutes.

Most commercial stage-top incubators that I've found and have personally used are meant for inverted microscopes and have a hole in the bottom for the objective and use the coverslip as the bottom window; I'm looking for a design that has a completely separate window on each side that would allow us to insert samples of up to a few mm thickness between the windows. Maybe someone in the light sheet imaging community would know of something similar?

Thanks a lot for any information you might have.

Cheers,
Kyle

Kyle M. Douglass - Engineer
EPFL SB IPHYS LEB
BSP 429 (Bâtiment Cubotron, UNIL)
CH-1015 Lausanne

Absent Wednesdays / absent les mercredis