Hello

Has anyone knowledge of a method to rapidly fix adherent cell cultures so
as to prevent the loss of small hydrophobic molecules?  The problem
involves subsequent diffusion of the antibody marker into the cytoplasm.
Material is examined using fluorescence/confocal microscopy  The organelles
of interest in this case are mitochondia but general recommendations would
be most appreciated too.

Regards

Andrew McNaughton

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Andrew McNaughton
South Campus Electron Microscope Unit
C/-Department of Anatomy and Structural Biology
School of Medical Sciences
University of Otago
PO Box 913, Dunedin
NEW ZEALAND

Telephone: +64 3 479 7308
Facsimile: +64 3 479 7254

e-mail: [log in to unmask]
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