Dear Michal:
I think you can try to do ratio Ca measurements in living cells using Fluo-
3 Am and Fura Red. More information:
-Novak,E.J.,Rabinovitch,P.S."Improved sensitivity in Flow Cytometric
Intracellular Ionized Calcium Measurement Using Fluo-3/Fura Red
fluorescence ratios". Cytometry 17:135-141 (1994).
-Schild,D. et al. Cell Calcium 15(5):341-8 (1994).
Susanna Castel
UNITAT DE MICROSCOPIA CONFOCAL
SERVEIS CIENTIFICO-TECNICS.UNIVERSITAT DE BARCELONA
LLUIS SOLE I SABARIS, 1-3 08028 BARCELONA
Tel. 34 3 402 13 52 Fax. 34 3 402 13 98
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