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This is what I meant when I said the regulations differ: for us, we just
need to have a sealed carrying container when we transfer our
virus-infected samples from the incubator room to our imaging room, and a
cleanup protocol.  We didn't need to have a sealed stage insert like Eric's
group.  Of course I'm not sure that Canada's L2 is treated the same as L2
in the US.

Craig


On Wed, Jan 4, 2012 at 3:07 PM, Eric Marino <[log in to unmask]> wrote:

> *****
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>
> To obtain BL2 approval from the university we had to submit a
> comprehensive clean-up and training protocol. We had to build a fully
> contained specimen stage insert that  allows the user to perfuse virus into
> a petri dish inside the stage insert without the risk of air-born
> contamination. We have to notify the other users of the core the type of
> pathogen that's being imaged that day.
>
> Eric Marino
> Senior Imaging Specialist
> Harvard Medical School / IDI
> 200 Longwood Ave
> WAB Room 133D
> Boston, MA 02115
> [log in to unmask] edu
>
> On Jan 4, 2012, at 3:59 PM, Terri Bruce wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
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> >
> > That's basically our policy at Clemson. We require the group to file a
> copy of their containment/clean-up protocol with me for review prior to
> using the facility. If I have questions and concerns regarding anything, I
> consult our campus IBS committee. I generally accept BSL2 but nothing
> higher in the core.
> >
> > Terri F. Bruce, Ph.D.
> > Research Assistant Professor
> > Manager, Jordan Hall Imaging Facility
> > Department of Biological Sciences
> > Clemson University
> > 132 Long Hall
> > Clemson, SC 29634
> > Phone: (864) 656-1264, FAX: (864) 656-0435
> > E-mail: [log in to unmask]
> >
> >
> > -----Original Message-----
> > From: Confocal Microscopy List [mailto:[log in to unmask]]
> On Behalf Of Cammer, Michael
> > Sent: Wednesday, January 04, 2012 3:36 PM
> > To: [log in to unmask]
> > Subject: Re: pathogens in a common use confocal core facility
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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> >
> > At two institutions I've been at it has been simple to accommodate BSL2
> but nothing higher.  The protocols call for containment of material and
> have an etoh and/or bleach clean-up procedures if the containment is
> broken.  This works well.
> >
> > Personally, I wouldn't want potentially more dangerous material in a
> standard core situation.  But there may be BSL3 and BSL4 facilities that
> have available microscopes (a few years ago I was invited to work on a TB
> project in one such facility, but declined).
> > ________________________________________________________
> > Michael Cammer, Assistant Research Scientist
> > Skirball Institute of Biomolecular Medicine
> > Lab: (212) 263-3208  Cell: (914) 309-3270
> >
> >
> > -----Original Message-----
> > From: Confocal Microscopy List [mailto:[log in to unmask]]
> On Behalf Of Phillips, Thomas E.
> > Sent: Wednesday, January 04, 2012 3:12 PM
> > To: [log in to unmask]
> > Subject: pathogens in a common use confocal core facility
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
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> >
> > I fully understand that use of pathogens requires approval of an
> institutional biosafety committee and that each circumstance is unique.
> Having said that, I am wondering if any of those of you who run a confocal
> core (or use pathogens in a core), have successfully implemented a
> biosafety plan which allows a client whose pathogens has "modest" risks
> could sporadically use an instrument that is widely used by other labs for
> no-risk, fixed samples or non-hazardous live samples. Is it crazy to try to
> accommodate this type of project or is it easier than it sounds? If you
> have experience where you got biosafety committee approval to bring a live
> pathogen into a core facility for imaging, I would be interested in knowing
> the type of agent was allowed and what you had to do to clean the room and
> instrument before it could be used by other clients.  Thanks and Happy New
> Year. Tom
> >
> >
> >
> >
> > Thomas E. Phillips, Ph.D
> > Professor of Biological Sciences
> > Director, Molecular Cytology Core
> > 2 Tucker Hall
> > University of Missouri
> > Columbia, MO 65211-7400
> > 573-882-4712 (office)
> > 573-882-0123 (fax)
> > [log in to unmask]<mailto:[log in to unmask]>
> >
> > http://www.biology.missouri.edu/faculty/phillips.html
> > http://www.biotech.missouri.edu/mcc/
> >
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