Hey Stu,

Buy a 10x/0.45 or Pete's sake!

Pete 

On Apr 29, 2010, at 12:50 PM, stu_the_flat wrote:

> Hello 
> 
> In our lab, we have a two-photon microscope that has 10x NA 0.3 and a 40x NA
> 0.8 objective. 
> 
> Recently some people in our lab have taken to using the 10x objective. I
> calculate the point spread function is going to be 18.5µm! That is not
> taking into account the tissue scattering effects (that I don’t know how to
> calculate) 
> 
> Can this set up just be thought of taking very broad optical section system
> or are there other things to take into consideration? 
> 
> Many thanks 
> 
> Stuart
> -- 
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