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Dear Michelle, 

   thank you for the list. Of course, I am aware about the methodological developments and their usefulness. I was not aware, however, of some of the most recent biological applications. Amongst others, the Cdc42 paper is clearly a very good example. It is not trivial for me to select the most topical biological discoveries where FLIM played a fundamental role.

Cheers, 

Alessandro
www.quantitative-microscopy.org