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AutoQuant is also an option, with measured and/or theoretic PSFs.
Forwarded from our sales folks:

>Dear Sarah,
>
>Deconvolution was something that originated in astronomy.  2D images
and 2D PSFs were all >that were used.  These approaches have been
applied to light microscopy and have been >expanded to the 3D case to
better improve the reassignment of out of focus blur.
>
>2D deconvolution with a 2D PSF is something that the AutoQuant
deconvolution package is >capable of handling.  The 2D deconvolution
significantly improves an image's signal to >noise, contrast and
resolution.  With the AutoQuant product you can use a theoretical PSF
>or measured PSF for deconvolution.
>
>Please visit http://www.mediacy.com/index.aspx?page=AutoDeblurVisualize
to request a fully >functional 30 day trial of our software.
>
>Best wishes with your imaging.
>
>Jonathan Girroir
>Software Product Manager
>
>Media Cybernetics
>731 NE Olney Ct. #1
>Bend, OR 97701
>cell 518.588.6729
>office 541.383.4133
>fax 541.383.4133

Kevin Ryan - Media Cybernetics, Inc.



-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Sarah Kefayati
Sent: Tuesday, March 11, 2008 8:54 PM
To: [log in to unmask]
Subject: 2D reconstruction

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Hello all:

I am collecting in situ psfs from Arabdiopsis leaves.For me it is
impossible to collect PSF's in z direction  as I will also capture the
leaf structure too and I only collect data in XY plane.
I am wondering if it is possible to do 2D deconvolution by these
collected PSF s with Huygens software(we haven't get the soft ware yet
we would like to try this with the test licence of the software).

I really appreciate any information and any idea.

Sarah