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August 2012

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Subject:
CFP and GFP proteins
From:
Ramana Sidhaye <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 1 Aug 2012 19:55:47 +0000
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Hi,
  One of my collaborators has a mouse endogenously expressing 2 proteins (both are expressed in the same cells), one coupled to CFP and the other to GFP. I am curious about the spectrum- how does one best evaluate both proteins in the same sample without concerns about cross-reactivity.

Thanks,
Ramana SIdhaye

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