Well, I know living cells hate blue light.  Not particularly helpful I
know, but flagellar axonemes crap out pretty fast with blue illumination
and last forever with red.  I suspect htat any muscle fiber would respond
similarly.
 
Try Calcium Orange or Red?
 
Tony  Moss
 
 
 
On Mon, 18 Jul 1994, Mike Ferrari wrote:
 
> I am using both a confocal setup and mercury arc with photoscope setup to
> image fluo-3 in Xenopus somitic mesoderm in culture. These myocytes seem
> particularly sensitive to illumination (dye loaded or not), and I am having
> difficulty getting post-imaging survival. Problem seems specific to mesoderm/
> myocytes as similar difficulty is not encountered in our lab with neuroectoderm/
> neuron cultures. Have limited illumination (e.g. via neutral density filters,
> long acquisition intervals, short imaging period, etc.) as much as feasible with
> no effect. Limited experimentation with antioxidants or free radical scavengers.
> Anyone imaging mesoderm or muscle cultures with similar problem(s)? Suggestions
> welcome and much appreciated.
>
> Mike Ferrari
> UCSD Bio Dept   [log in to unmask]
>