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>Regarding DF at NA>1.0, be sure to use an oil objective with a built-in
>iris.
>

Actually, as we were talking cell movement, I assumed living cells
and that one would be using a water-immersion lens. These normally
don't go above NA 1.2.

On the other hand, the maximum effective NA of the condenser (BF or
DF) would be less than the 1.4 marked on it because of the water's
lower RI. I am not away of any condensers designed specifically for
water-immersion.

Cheers,

Jim P.
--
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Prof. James B. Pawley,                             Ph.  608-263-3147
Room 223, Zoology Research Building,               FAX  608-265-5315
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"A scientist is not one who can answer questions but one who can
question answers."  Theodore Schick Jr., Skeptical Enquirer, 21-2:39