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June 2020

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Subject:
Re: Mounting tissue section onto the coverslip
From:
Sylvie Le Guyader <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 24 Jun 2020 08:28:33 +0000
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Hi again

You are very welcome to use our videos.
My experience is that it is easy to convince our users to put their sample on the coverslip. When we train them, they must prepare samples for the training. We simply tell them to make 2 samples in parallel, one the way they normally do it and one identical but on the coverslip. It takes no extra effort so they do it.
Then we show them the difference with a high NA objective and let them decide what they want.
One only sees the effect of not preparing the sample in an optimal way when one looks at the images side by side. 😊

Med vÀnlig hÀlsning / Best regards
Sylvie
@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager
Karolinska Institutet- Bionut Dpt
BlickagÄngen 16,
Room 7362 (lab)/7840 (office)
14157 Huddinge, Sweden
mobile: +46 (0) 73 733 5008
LCI website
LCI microscopy blog

-----Original Message-----
From: Erika Wee <[log in to unmask]>
Sent: Tuesday, 23 June, 2020 19:03
To: [log in to unmask]; Sylvie Le Guyader <[log in to unmask]>
Cc: Erika Wee <[log in to unmask]>
Subject: Re: Mounting tissue section onto the coverslip

Dear Sylvie,

Thank you so much for the detailed into, and also the YouTube videos. I agree with you 100% that samples should be always mounted onto the coverslip side. I just did a microscopy workshop last week, I find it's really difficult to ask people to modify their existing sample prep protocol to mount the sample to the coverslips.

The YouTube videos are really well made and super helpful, especially I really can't go to each lab to show them how to work on microscopy samples. I just forward the info you provided to my lab and facility users to watch it.

Thank you so much!

Best,
Erika



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