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April 2012

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Subject:
From:
Johannes Schindelin <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Sun, 15 Apr 2012 00:24:44 -0500
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Hi Mark,

On Sun, 15 Apr 2012, Mark Cannell wrote:

> The point is that the pixels integrate over their entire face which is
> square so that this may be represented by square pixels perfectly
> faithfully.

Point is: at the resolution we're operating, PSFs play a role. It does not
matter whether the detector is square. The non-square part happens earlier
in the process, the detector cannot undo it.

All you can safely assume of a pixel is that it has one or more values
(even referring to the value as an "intensity" need not be correct) and
that it has a center. The rest depends on the setup.

Ciao,
Johannes

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