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Will this system have the same sort of sensitivity as a multi-anode
PMT system like the C1si?  This PARISS system seems to use a CCD
camera as its detector, which will not be as sensitive, if I
understand correctly?

Craig

On 10/5/07, Robert Zucker <[log in to unmask]> wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Criag
> If spectral analysis is the endpoint you may want to consider the PARISS
> system from Lightform. ( http://www.lightforminc.com/) that can be
> placed on a widefield microscope.
> We are using the PARISS system to obtain spectral data --it has 1nm
> resolution and has a spectrum from 400-900nm. I find it to be a great
> asset in the laboratory,
> It is far more sensitive and accurate that any confocal system that I
> have seen.
>
> Contact Jeremy Lerner for details on his system.
> best wishes
> Bob
>
>
>
>
>
>
>
>
>                                 LightForm, Inc.,
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>
>
>
> .
>
> Robert M. Zucker, PhD
> U.S. Environmental Protection Agency
> Office of Research and Development
> National Health and Environmental Effects Research Laboratory.
> Telephone: 919-541-1585   Fax: 919-541-4017
> e-mail: [log in to unmask]
>
> Mail address: Reproductive Toxicology Division, MD 67
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>
>
>
>              Craig Brideau
>              <craig.brideau@G
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>              RV.BUFFALO.EDU>          Re: Nikon C1si?
>
>
>              10/05/2007 05:48
>              PM
>
>
>               Please respond
>                     to
>                  Confocal
>              Microscopy List
>              <CONFOCAL@LISTSE
>              RV.BUFFALO.EDU>
>
>
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>
> Search the CONFOCAL archive at
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>
> Thanks for your input!  As in everything, there's a trade-off.  Still,
> for us the spectral data will be worth it.
>
> Craig
>
> On 10/5/07, Robert Zucker <[log in to unmask]> wrote:
> > Search the CONFOCAL archive at
> > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >
> > Craig
> > The efficiency of detection is much less in the spectral mode due to
> the
> > design of the multianode detector. In addition as Kurt Thorn said the
> > light is split into a number of different bandpass channels instead of
> > being detected by only one channel which also limits the detection in
> > each channel.
> > Nikon compensates for this decreased light by increasing the pixel
> dwell
> > time. However photons are photons. With less photons you will get a
> > noisier image.
> > The spectral detection of any confocal system using a multianode
> > detector will not be as good as a PMT designed for good sensitivity
> and
> > low light detection. It will produce nosier images but if there is
> > enough light you will be able to determine a valuable spectrum which
> can
> > be used for scientific experiments and to manipulate images. Like
> > everything with confocal microscopy there is trade-offs and no perfect
> > system.
> > Best wishes.
> > Bob
> >
> > Robert M. Zucker, PhD
> > U.S. Environmental Protection Agency
> > Office of Research and Development
> > National Health and Environmental Effects Research Laboratory.
> > Telephone: 919-541-1585   Fax: 919-541-4017
> > e-mail: [log in to unmask]
> >
> > Mail address: Reproductive Toxicology Division, MD 67
> > 2525 E.NC Highway 54
> > Research Triangle Park, North Carolina, 27711
> >
> > Shipping address: 2525 E.NC Highway 54
> > Durham, NC, 27713
> >
> >
> >
> >
> >              Craig Brideau
> >              <craig.brideau@G
> >              MAIL.COM>
> To
> >              Sent by:                 [log in to unmask]
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> Subject
> >              RV.BUFFALO.EDU>          Re: Nikon C1si?
> >
> >
> >              10/04/2007 02:49
> >              PM
> >
> >
> >               Please respond
> >                     to
> >                  Confocal
> >              Microscopy List
> >              <CONFOCAL@LISTSE
> >              RV.BUFFALO.EDU>
> >
> >
> >
> >
> >
> >
> > Search the CONFOCAL archive at
> > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >
> > I was wondering if you saw this decreased throughput in spectral mode
> > only, or also in conventional detection mode?  The spectral mode works
> > with a multi-anode PMT, which has a lower quantum efficiency than a
> > conventional PMT.  From what I have seen of the design, it also has
> > the option to use a conventional PMT system with the device, and I am
> > wondering if you have also tried that mode of operation?
> >
> > Thanks,
> >
> > Craig
> >
> > On 10/4/07, Robert Zucker <[log in to unmask]> wrote:
> > > One of the major limitations that we have seen with this unit and
> also
> > > with  the Zeiss meta 510 is the decreased light throughput . This
> > > creates images that are noisier than conventional confocal
> > microscopes.
> > > In our hands it appears you will need to have a bright sample to
> make
> > > the spectral system work properly. ,
> >
>